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Last updated: October 2, 2026

The nitrogen cycle: from air to life

Nitrogen cycle illustrating fixation, nitrification, assimilation and denitrification

The biogeochemical nitrogen cycle unfolds in several major steps: atmospheric nitrogen is first fixed, notably by bacteria and lightning; it is then assimilated by plants, then returned to the soil through decomposition, before finally returning to the atmosphere.
Image source: astronoo.com (new window) - AI-generated image, public domain.

Scientific summary

The Astronoo article presents the nitrogen cycle, a set of biogeochemical transformations that make atmospheric nitrogen assimilable by living organisms. Dinitrogen (N₂) makes up about 78% of the air, but its triple bond renders it almost inert. Nitrogen-fixing bacteria, fungi and physical phenomena such as lightning convert this gas into ammonia, then into nitrites and nitrates. Plants absorb these compounds to build proteins and DNA. Decomposition and denitrification close the cycle by returning nitrogen to the atmosphere. The article also highlights human disturbances: synthetic fertilizers, eutrophication and N₂O emissions.

Why is the nitrogen cycle essential to life on Earth?

Nitrogen is an essential element for building proteins, amino acids and DNA. Yet Earth's atmosphere, which contains nearly 78% of it as dinitrogen (N₂), remains a resource inaccessible to most living beings. The N₂ molecule is stabilized by a triple chemical bond that is very difficult to break. The nitrogen cycle responds precisely to this paradox: it continuously transforms atmospheric nitrogen into assimilable forms, thanks to nitrogen-fixing bacteria, fungi, plants and natural phenomena such as lightning. Fixation produces ammonia, which is then transformed into nitrites and then nitrates through nitrification. Plants absorb these compounds and integrate them into their living matter; animals obtain them by consuming plants. Decomposition, ammonification and denitrification close the cycle by returning nitrogen to the atmosphere. Without these conversions, ecosystems could not maintain their fertility, ocean productivity would be profoundly altered and climate stability would be threatened. The nitrogen cycle is therefore an invisible but fundamental cog of the biosphere.

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Why is nitrogen, although so abundant, so difficult to capture?

A colossal but inert atmospheric stock

Earth's atmosphere contains nearly 78% dinitrogen (N₂) by volume, or 75% by mass. Yet this enormous potential resource for life remains inaccessible to the vast majority of living beings. No animal, no human, no plant can transform it directly into a nutrient.

The triple bond, lock of the N₂ molecule

The reason lies in the exceptional robustness of the triple chemical bond between the two nitrogen atoms (N≡N). Considerable energy is required to break this bond.

Two pathways to break the molecule

Two pathways exist: lightning and its 30,000 °C, or the chemical elegance of bacteria and their enzyme nitrogenase, capable of reducing N₂ to NH₃ (dinitrogen → ammonia). Without this prior rupture, no proteins, no DNA, no muscles, no neurons, no blood: no life.

A cycle in the service of the living

The nitrogen cycle is a cascade of transformations that converts an inert gas into assimilable nutrients, then returns it to the atmosphere.

N.B.:
Antoine Lavoisier (1743-1794) proposed the term "azote" (from the Greek "a-zôê", without life). Paradoxically, this element, inert in gaseous form, proves indispensable to every living cell once it is fixed.

Which microorganisms for which transformation?

The table below compares the major pathways of the nitrogen cycle and their biological actors.

Specialized microbial actors

Comparison of the major pathways of the nitrogen cycle and their biological actors
ProcessChemical transformationBacteria + FungiRequired conditionsEcological role
Symbiotic fixation\(N_2 \rightarrow NH_3\)Rhizobium, BradyrhizobiumSymbiosis with legume roots, microaerophiliaNitrogen supply directly to cultivated plants
Free-living fixation\(N_2 \rightarrow NH_3\)Azotobacter (aerobic), Clostridium (anaerobic)Soils, aquatic environments, available organic carbonDiffuse but constant contribution to natural fertility
Nitrification (step 1)\(NH_3 \rightarrow NO_2^-\)Nitrosomonas, NitrosospiraStrict aerobiosis, neutral to slightly alkaline pHFormation of nitrites, precursors of nitrates
Nitrification (step 2)\(NO_2^- \rightarrow NO_3^-\)Nitrobacter, NitrospiraAerobiosis, tolerance to a wide range of temperaturesProduction of nitrates, a highly assimilable form for plants
Denitrification\(NO_3^- \rightarrow N_2\) (via \(NO_2^-\), NO, \(N_2O\))Pseudomonas, Paracoccus, BacillusAnoxia (saturated soils, sediments), labile organic matterClosing of the cycle, return of \(N_2\) to the atmosphere
Ammonification (mineralization)Organic N \(\rightarrow NH_4^+\)Saprophytic fungi (Bacillus, Streptomyces, etc.)Aerobiosis or anaerobiosis, decomposition of necromassRecycling of nitrogen from organic waste

N.B.:
Human activity through synthetic fertilizers has doubled the flows of reactive nitrogen entering terrestrial ecosystems since 1950.

When humans unbalance the nitrogen cycle

A rupture of equilibrium on a planetary scale

Before the industrial era, natural flows of biological and atmospheric fixation maintained a stable stock of reactive nitrogen in soils and waters. The work of Justus von Liebig (1803-1873) had already foreshadowed the importance of nitrogen compounds for fertility. But it was the invention of the Haber-Bosch process (first decade of the 20th century) that multiplied the production of synthetic nitrogen fertilizers. Today, anthropogenic fixation exceeds natural fixation.

Eutrophication and greenhouse gases

Excess nitrates leached by rain reach rivers and then coastlines, causing eutrophic dead zones where algal blooms asphyxiate aquatic fauna. The Gulf of Mexico and the Baltic Sea illustrate this diffuse pollution. Furthermore, nitrous oxide (N₂O), a by-product of nitrification and denitrification, is a greenhouse gas 300 times more powerful than carbon dioxide, contributing to global warming.

References

FAQ: Nitrogen cycle

What is the nitrogen cycle?

It is the set of transformations that allow nitrogen to pass from its atmospheric form to forms usable by living organisms, then return to the atmosphere.

Why is atmospheric nitrogen not directly usable?

Because organisms cannot break the N₂ molecule, which is very stable. Only certain bacteria are capable of fixing it.

What is the role of bacteria in this cycle?

They carry out the key steps: fixation, nitrification, ammonification and denitrification, making nitrogen accessible or returning it to the atmosphere.

How do plants use nitrogen?

They absorb nitrates and ammonium present in the soil to build proteins, amino acids and DNA.

How do human activities disrupt this cycle?

Fertilizers, pollution and industrial combustion add too much reactive nitrogen to the environment, causing eutrophication, ecological imbalances and greenhouse gas emissions.

How can we protect or rebalance the nitrogen cycle?

We must reduce excess fertilizers, improve water treatment, preserve wetlands and living soils, and monitor N₂O emissions. These actions limit eutrophication and warming.

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